Proteolytic Enzymes Formula | Natural Health Newsletter

Date: 08/26/2006    Written by: Jon Barron

A Proteolytic Enzyme Formula

In this alternative health newsletter I want to introduce you to a new concept in proteolytic enzymes. This represents a reformulation of my original concept for proteolytic enzymes by combining a unique pH buffering system with an upgrade in a key enzyme. The formula is so powerful, so unique, and so effective that it has been granted a U.S. patent. For those of you who are familiar with proteolytic enzyme formulas, you can skip down to "Why pH matters to enzymes." For the rest of you, before I tell you about the innovations within this new proteolytic enzyme formula, let's review the basics on systemic, proteolytic enzyme formulas -- how they help with cellular metabolism, how they breakdown viruses, bacteria, and proteins, and much more.

Proteolytic enzymes 101

What are enzymes?

Enzymes are proteins that speed up (catalyze) chemical reactions in living organisms. In fact, they are required for every single chemical action that takes place in your body. Enzymes run all of your cells, organs, bones, muscles, and tissues.

Enzymes allow many chemical reactions to occur within the constraints (temperature, oxygen levels, acid/alkaline balance, etc.) of a living system. As organic catalysts, they are involved in, but not changed by, chemical reactions; and they do not alter the equilibrium of those reactions.

Like all catalysts, enzymes work by providing an alternative pathway of lower activation energy for a reaction. By bringing the reactants closer together, enzymes can help make chemical bonds weaker, thus helping reactions proceed faster than without the catalyst; making the reaction many millions of times faster. This is important since these "reactions" govern every function in your body, not to mention the destruction of viruses, bacteria, and cancerous cells.

Proteolytic Enzymes

Without enzymes, metabolism would progress through the same steps, but would go too slowly to serve the needs of the cell. In addition, enzymes often work together in a specific order, creating pathways. After each enzyme reaction, the product of that reaction is passed on and used as the raw material (substrate) for another enzyme to work on. After each enzyme reaction, the product of that reaction is passed on and used as the raw material (substrate) for another enzyme to work on. Metabolic pathways control cell metabolism. Cell metabolism is the process (or really the sum of many individual processes) by which living cells take in nutrients, eliminate waste, and maintain life.

In essence, enzymes are the stuff of life. By allowing reactions to happen at far lower energy thresholds, they make life happen where otherwise there would be none. In the movies, Victor Frankenstein used electricity to create life; he probably would have been better served using enzymes.

Beyond digestion

An important concept to understand is that digestive enzyme formulas and systemic/metabolic proteolytic enzyme formulas, although they may share many of the same enzymes, are not the same thing.

  • When you take a digestive enzyme formula with your meal, the enzymes work on speeding up the breakdown of the food in your stomach.
  • When you take a proteolytic enzyme formula between meals, the enzymes do not get stuck working in your stomach. Instead, they quickly enter your bloodstream. In the bloodstream, they can help optimize your blood, plus they can also travel to tissues throughout your body to assist with intelligent, adaptive healing.

Understanding proteolytic enzymes

The vast majority of metabolic enzymes in the body, the enzymes that regulate everything from liver function to the immune system, are proteases, or proteolytic enzymes -- the focus of this alternative health newsletter. Proteolytic is a catchall phrase for enzymes that specifically facilitate the chemical breakdown of proteins by breaking the bonds between the amino acids that make up those proteins.

Proteolytic enzymes occur naturally in all organisms and constitute 1-5% of the gene content. They are polymorphic. The enzyme action they produce can adapt to meet the current digestive or metabolic needs of the body. In other words, proteolytic enzymes are able to adapt to the specific needs of their environment, plus they do not harm any healthy cells. Normal living cells are protected against destruction by the inhibitor mechanism.

Proteases are involved in a multitude of physiological reactions from simple digestion of food proteins to highly regulated cascades (e.g. the blood clotting cascade, the complementary immune system, apoptosis activation cascade and the invertebrate prophenoloxidase activating cascade).

The bottom line is a healthy supply of these protein specific enzymes is essential for sustaining and maintaining optimal health.

Proteolytic enzymes and illness

Just about everything that makes us sick is either a protein or is protected by a protein and is therefore subject to control by enzymes.

  • Bacteria, viruses, yeasts, and fungi are all protected by proteins.
  • Food allergens are all proteins.
  • Cancer cells are protected by proteins.

Proteolytic enzymes have the ability to digest and destroy the protein based defense shield of each and every one of these pathogens, thereby leading to their ultimate elimination.

And then there are CIC's (Circulating Immune Complexes). CIC's start out as extra-large protein molecules (primarily from wheat, corn, and dairy) that are only partially digested in the small intestine and are absorbed into the bloodstream. Once in the bloodstream, the immune system treats them as invaders because they are too large to be metabolized, provoking an immune reaction. Antibodies couple with these foreign protein invaders to form CIC's. At first, these CIC's may be neutralized in the lymphatic system. But over time, as too many CIC's are created, they overwhelm the body's ability to eliminate them. They overwhelm the immune system, and they overwhelm the kidneys. At that point, the body has no choice but to "store" them in its soft tissues, where the immune system continues to attack them as allergens causing inflammation and, ultimately, autoimmune disorders.

It is here that proteolytic enzyme supplements come into play. They compensate for your dietary inadequacies and errors by making their way into your bloodstream, where they set to work breaking down CIC's in your blood and soft tissues -- eventually passing the waste out through your kidneys.

And finally, some proteolytic enzymes such as nattokinase and endonase are very specialized. They work on specific protein related tasks such as optimizing blood, reducing pain and inflammation, and cleaning out the lungs.

Why you need to supplement?

In a perfect world, we would all eat raw (organic) unprocessed foods that are naturally high in active enzymes.

Note that man is the only animal that cooks his food (or eats processed foods for that matter). Cooking and processing kills enzymes. In fact, any sustained heat of approximately 118' F destroys virtually all enzymes. This means that, for most of us, the food entering our stomachs is severely enzyme deficient.

But this isn't a perfect world.

Most people eat food that is enzyme deficient and fail to chew food adequately so their bodies must compensate. The body is forced to divert its production of enzymes away from proteolytic enzymes, which govern metabolic functions, into digestive enzymes designed to break down dead proteins in our diets. The consequences of this diversion are enormous. It can lead to everything from a weakened immune system to thickened blood, from pain and inflammation to cardiovascular disease, and from difficulty breathing to reduced athletic performance. Supplementing with proteolytic enzymes offsets this devastating diversion.

Why pH matters to enzymes

As I mentioned at the top of the newsletter, the big breakthrough in this new enzyme concept is in the addition of a pH buffering system. But why does pH matter to enzymes?

Although pH is in actuality a measure of the degree of Hydrogen (H) ions in a substance or solution, it is best recognized as a measure of the acid/alkaline balance of anything. (0-7 is acid; 7 is neutral; 7-14 is alkaline or base.) In another sense, though, it is also a measure of the available (unbound) oxygen in something -- the higher the pH, the more available oxygen there is in your body. A proper alkaline environment in your blood provides literally thousands of times the oxygen needed for all bodily functions. Proper pH by itself is a major factor in optimizing health and disease prevention (cancer, for example, thrives in an acid environment), but it also directly impacts the efficacy of proteolytic enzymes in your body.

"Enzymes function optimally at a specific pH and become inactive if this deviates beyond narrow limits," - Anatomy and Physiology by Anthony and Thibodeau.

Think of it this way. There are hundreds if not thousands of enzyme processes that take place in the body. Many are so specific that they are like complex square pegs that need to "fit" into specific square holes in order to carry out their function. If pH is off-balance even a little, some important pegs will not "fit" into their respective slots. Enzyme function and thus life itself begins to suffer.

Not surprisingly then, different pH's are required to optimize different body functions. For example, your saliva is slightly alkaline, whereas your stomach juices are strongly acidic. Your intestines operate best in a strongly alkaline environment, and your urine prefers a more acidic environment for control of bacteria. The most important pH of all, however, is your blood pH, because a deviation of as little as 5/10th of a point from its normal pH of 7.45 can result in illness or death.

That said, small deviations of less than 1/10th of a point can make a huge difference in your health and in the effectiveness of the proteolytic enzymes that operate in your blood. The bottom line is that, in general, the higher the pH in your blood, the more active the enzymes. This has implications for everything from boosting your immune system to your ability to utilize oxygen.

When it comes to proteolytic enzymes, most proteolytic enzymes (other than pepsin) work best in a neutral to slightly alkaline environment. Optimizing that environment, then, becomes crucial for optimal health.

What changes your body's pH?

When you metabolize carbohydrates, proteins, and fats, various acids are produced. Proteins, for example, produce sulfuric acid and phosphoric acid. Carbohydrates and fats produce acetic acid and lactic acid. The bottom line is that diet changes your body's pH. And most of us eat a highly acidic diet (meat, dairy, cooked grains, and most bottled drinks).

Why Minerals are important.

There are complex biochemical processes constantly working to keep your pH levels optimized for maximum enzyme activity. These are known as pH buffering systems. They are mineral based. If you lack minerals in your diet (common in modern diets), then your body's natural buffering system is deficient.

Essentially, your body uses minerals such as sodium, calcium, potassium, and magnesium to combine with the acids and turn them into pH neutral mineral salts. These salts can then be safely eliminated through your kidneys.

An enzyme epiphany

We have talked about how important an optimal pH environment is to the effectiveness of proteolytic enzymes. CMIK-pH+ (a proprietary blend of calcium, magnesium, ionic trace minerals, and potassium that has been added to this new proteolytic enzyme formula) creates just such an environment -- and a whole lot more.

  • First, it provides extra protection for the enzymes from stomach acid. Normally, if you take your proteolytic enzymes on an empty stomach, the acid content is fairly low so they don't suffer much damage anyway -- particularly if you choose your enzymes carefully. This was one of the reasons for using Seaprose-S instead of serrapeptase in the formula. Nevertheless, it provides that "extra level" of protection. (Note: enteric coating is a pretty marginal technology when it comes to capsules. It doesn't really protect the enzymes very well.)
  • It helps optimize the pH of the blood. Again, as said previously, blood pH will vary very little to protect against death. However, as also stated, minor changes in blood pH make a huge difference in proteolytic enzyme efficiency and oxygen availability. The impact of CMIK-pH+ in this regard is monumental.
  • It helps optimize the pH of all your soft tissue. Since your body will do anything to keep your blood's pH in a narrow range, that means it robs Peter to pay Paul. It steals minerals and lowers pH in your soft tissue to protect your blood if your diet is too acidic. And if you eat meat, dairy, cooked grains, desserts, and most fruit, then your diet is indeed too acidic. Bottom line here is that by raising soft tissue pH, you dramatically improve the ability of proteolytic enzymes to root out CIC's embedded in that soft tissue, thus relieving allergies and reducing the possibility of autoimmune conditions.
  • And finally, by providing a source of buffering minerals, CMIK-pH+ reduces the chances of osteoporosis. After all, if your body needs minerals to buffer a high acid diet, what's the biggest mineral bank in your body? Your bones! The presence of CMIK-pH+ can help offset some (or all) of that need, depending on how many capsules of the formula you take, so you keep minerals in your bones for strength.

The addition of CMIK-pH+ to a proteolytic enzyme formula is so revolutionary and so effective, it is now patent pending.

Upgrade in key enzyme

We have talked before about the powerful ingredients used in the original Proteolytic Enzyme formula. In this newsletter I want to update you on a change we made in one of the key enzymes, Seaprose-S.

Seaprose-S was integral to the original formula because of its remarkable anti-inflammatory and anti-fluid retention activity. In addition to reducing inflammation, it has a profound ability to reduce pain, clear mucous from the lungs, and break down sputum in patients with chronic airway diseases. And unlike serrapeptase, it is not affected by stomach acid so it does not require enteric coating. It is also much less upsetting to the stomach.

But from a manufacturing point of view, it is like a high-strung racehorse -- difficult to work with. So I looked for an alternative (for over a year) before discovering Endonase (aka Protease-S).

Endonase has all of the benefits of Seaprose-S (and is in fact closely related to it), but offers the advantage of being less concentrated so it's much easier to work with in manufacturing, resulting in a much more consistent final formulation.

Bottom line: Endonase allows us to get all of the benefits of Seaprose-S, but with much more precise tuning. For that reason, I replaced Seaprose-S with Endonase in the new formulation.

Proteolytic enzymes and optimal health

With an understanding of how your body utilizes proteolytic enzymes, what interferes with their potential (an acid body), and what maximizes their potential (an alkaline body), it becomes clear that the advantages of supplementing with a good systemic proteolytic enzyme formula with an added pH buffering system are profound. Possible benefits include:

  • Reduce inflammation for: Increased heart health, Cancer and disease prevention and recovery, Alzheimer's prevention, Fibromyalgia, Chronic fatigue, and more
  • Cleanse the blood of debris
  • Break down and remove Circulating Immune Complexes
  • Dissolve arterial plaque
  • Dissolve fibrin in the blood, reducing the risk of clots
  • Eliminating the risk of DVT (Deep Vein Thrombosis)
  • Boosting the immune system
  • Kill bacteria and viruses
  • Improve circulation
  • Eliminate autoimmune diseases
  • Reduce the risk of and response to food and pollen allergies
  • Speed up recovery from sprains, strains, fractures, bruises, contusions, and surgery
  • Speed up recovery time from workouts
  • Help with MS
  • Help with arthritis
  • Remove plaque from teeth
  • Help with sinusitis and asthma
  • Dissolve scar tissue
  • Aid in cleansing and detox fication
  • Improved body alkalinity
  • Reduced risk of osteoporosis


From removing dental plaque to protecting your cardiovascular system, from reducing inflammation throughout the entire body to speeding up the recovery times of athletes, the bottom line is that supplementation with a good proteolytic enzyme formula such as we've discussed here today is essential.

In addition:

  • Add more raw foods to your diet.
  • Cut out (or at least cut down) processed foods.
  • Cut out (or at least cut down) cooked foods.
  • Chew your food properly.
  • Drink alkalinized water.


A couple of years ago, we issued a challenge to dentists to validate the ability of systemic proteolytic enzymes to dissolve plaque. As far as I know, no dentist has ever taken up the challenge -- but some of our readers have

"Five months ago I had my teeth cleaned. At that time I had the expected, usual amount of plaque on my teeth. So the hygienist cleaned it up. One month ago I began taking the Proteolytic Enzymes for some joint inflammation I was experiencing after taking a round of antibiotics for a strong kidney infection. With the help of the Proteolytics, the pain went away very quickly, as I thought it would. Then 2 days ago, I went to the dentist again to have my teeth cleaned and sealed. The hygienist said I did not need my teeth cleaned because there was no plaque on my teeth at all!"
Caitlin W., OH

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    Submitted by Diana Santa Maria on
    April 17, 2011 - 9:49pm

    If I take Digestive enzymes with food, do I need to also take the Proteolytic Enzymes? Also, my son, who is 19 yoa has congenital liver fibrosis. I give him Digestive Enzymes with each meal. Should I give him the Proteolytic Enzymes as well or in lieu of the Digestive Enzymes? Thanks in advance for your reply. DSM

    Submitted by Richard Lizotte on
    January 1, 2012 - 11:27pm

    Looking for the answer to the question.

    Submitted by BaselineFoundation on
    January 2, 2012 - 10:43am

    Richard, Just scroll down to see answer. See this note: "Submitted by BaselineFoundation on April 18, 2011 - 7:57am."

    Submitted by Guest on
    April 18, 2011 - 8:03am

    Thank you for the article. There are tons of articles on intergrative health and alternative therapies. The problem is which brand of vitamin to use. How does the consumer know which brand actually has the appropriate amount of active ingredients in it? does cost effect the potency? the cost is sometimes prohibited for some brands, so does paying more money ensure quality. Do you have any suggestions on excellent brands of supplements that work? Thank you, A guest

    Dear Guest, there are a few products Jon recommends here:

    Submitted by BaselineFoundation on
    April 18, 2011 - 9:57am

    Hi Diane,

    Digestive enzyme formulas and proteolytic enzyme formulas share similarities in compositions, but are very different in purpose. Digestive enzymes are designed to be taken with meals to aid in the digestion of those meals. Proteolytic enzymes, on the other hand, are designed to be taken without food. You do not want them used up digesting food. You want them to enter the bloodstream and work there. Taking one does not eliminate the need for the other. For a complete explanation, read Jon Barron’s three part series on enzymes.

    As for your son, for obvious legal reasons, we cannot diagnose or prescribe for specific medical conditions. But again, in non-medical terms, taking digestive enzymes with food is different from taking proteolytic enzymes between meals. Again, Jon’s series of newsletters on enzymes will explain the difference.

    Submitted by janice gibbs on
    April 18, 2011 - 10:29am

    hi ......just wondering if am taking aspirin cos have small vessel taking 2 which i really don't want to or like doing.......what would be the recommended doseof the enzyme formula to equate with 2 daily aspirin.......if 10 or 12 tabs is the alternative enzyme dosing it would be for me, not possible, as i could not afford as i would be going through approx 3 - 4 bottles a month.....but if a lower dose
    (which i think you hint at as it is very effective) is recommended i totally would love to change to it.....thanks janice....

    Submitted by Sandy on
    May 30, 2011 - 8:50am

    I recently moved to New Mexico and found that in the early spring when the pollen as active from the Juniper trees I have serious allergies (itchy eyes, sneezing, scratchy throat, inflamation under eyes and some throat, runny to stuffy nose, fatigue). After the blooming passes the symptoms subside. I am doing a colon cleanse and considering the Phase 2 and 3 cleanse. Is juniper in the phase 2 or 3 detox formulas and if so would taking the formula be supportive or could it trigger allergy symptoms when taken internally? Thanks so much.

    Submitted by Brian on
    June 15, 2011 - 7:37pm

    Will proteolytic enzymes assist in reducing surgical adhesions as a result of abdominal surgery. I saw a comment they work on scar tissue.

    Submitted by Guest on
    June 25, 2011 - 3:27pm

    can children as young as 12 years old take proteolytic enzymes to help with asthma and sinus ??

    Submitted by BaselineFoundation on
    June 27, 2011 - 12:51pm

    Some children, with their pediatrician’s permission and guidance, do take proteolytic enzymes with beneficial results. You do want to have your pediatrician monitor the dosage since one of the benefits of proteolytic enzymes is that they decrease the tendency of blood cells to clump together. At the right levels, this is positive, but if the dosage is too high it can lead to a reduced ability of blood to clot – and in a smaller child’s body, that can happen at lower dosages.

    Submitted by maxwell on
    February 12, 2012 - 1:30pm

    this is one of the few examples of BF responses that refer to "the right dosage" for proteolytic enzymes which is a subject close to my heart (forgive the pun).. clearly one can expect different outcomes from supplementation depending on dosage. You say as much in your above response. So where might that parent find advice to avoid overdosing her child? Whilst recognising the ban on prescription which applies to JB et al I cannot help but conclude that the time is nigh when those who profit from supplementation products need take more responsibility for their application. In effect i guess i am suggesting that Jon should find a way to put his mouth where his money comes from when it comes to guidance on dosing for the supplements he designs and causes to be manufactured.. max

    Submitted by BaselineFoundation on
    February 13, 2012 - 10:18pm

    For obvious legal reasons, we cannot diagnose or prescribe. To name a particular dosage in this case would amount to prescribing for a child. We can list general guidelines for nutrients as “nutritional support” supplements for adults, but cannot recommend specific dosages for children. However, most pediatricians use the weight method for prescribing supplements for children. Assuming an average adult weight of 150 lbs, then a child of 75 lbs would get half the adult dose. A child of 50 lbs would get 1/3 the dose. Check with your pediatrician to see if they use that method for calculating supplement doses for your child.

    Submitted by Guest on
    July 9, 2011 - 2:30am

    What should be the recommended daily amount of Protease? i was wanting to use it to cleanse my blood free from toxins

    Submitted by BaselineFoundation on
    July 11, 2011 - 10:01am

    That question as phrased doesn’t actually have meaning. Protease is a general description for any enzyme that breaks down proteins. It’s a bit like asking, “What would be the recommended daily amount of vegetable.” It depends on what specific type of proteases you use, what’s their strength, how they’re combined in a formula, etc.  For instructions on how to use such a formula, check out Keep in mind that different formulas, will be used differently.

    Submitted by George Ul. on
    July 11, 2011 - 10:24am

    Dear Dr. Barron,

    what is your input about the "Pauling Therapy" which recommends massive amounts of Vitamin C daily, to reverse arteriosclerosis.
    Does it work, or is it another idea that does nothing.



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